biological source
human pleural cavity
packaging
tube of 5 μg 12050301-DNA-5UG, pkg of vial of cells 12050301-1VL
growth mode
adherent
morphology
Epitheloid/mesenchymal
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
STR-PCR Data:
Amelogenin: X
CSF1PO: 9,11
D13S317: 8
D16S539: 12
D5S818: 10,13
D7S820: 7,11
THO1: 6
TPOX: 8
vWA: 15,19
Amelogenin: X
CSF1PO: 9,11
D13S317: 8
D16S539: 12
D5S818: 10,13
D7S820: 7,11
THO1: 6
TPOX: 8
vWA: 15,19
ZL55 was derived from an epithelial tumor of the pleural cavity of a 52-year old male, prior to treatment, with a known history of exposure to asbestos.
Preparation Note
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 15% Fetal Bovine Serum (FBS)
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 1-4 x 10,000 cells/cm².
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.