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About This Item
UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgG1
Technique(s):
cell culture | mammalian: suitable
Myeloma:
NS1
Reactivity:
Human CD200R
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 13082222-DNA-5UG, pkg of vial of cells 13082222-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
NS1
Reactivity
Human CD200R
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Recombinant CD200R
Application
Detection of CD200R (gives better binding than OX-109).
Biochem/physiol Actions
The hybridoma cell line OX-108 secretes monoclonal IgG1 antibodies reactive with human CD200R.
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Seed at 5 x 104 cells/ml (5 x 105 cells/ml at resuscitation). Subculture when cells reach 5 x 105 - 1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. 20% FBS can be used at resuscitation.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Regulatory Information
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