KHOS/NP
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human bone
packaging
tube of 5 μg 84102903-DNA-5UG
pkg of vial of cells 84102903-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Assay of type-C leukaemia viruses.
Biochem/physiol Actions
Human Caucasian osteosarcoma
Isolated from transformed foci of human osteogenic sarcoma (HOS) cells which were induced by Kirsten murine sarcoma virus. They contain MSV genome but do not produce infectious virus. The transformed cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumours in nude mice.
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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