biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 84112015-DNA-5UG, pkg of vial of cells 84112015-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
NS1
Reactivity
Anti-MHC Class I (polymorphic), rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
Rat PHA blast cells
Biochem/physiol Actions
Mouse NS1 x Mouse BALB/c spleen
The antibody is directed against an MHC Class I determinant which is polymorphic in the rat (RT-1Ac+, RT-1Au-, RT-1Al-, RT-1An+).
Preparation Note
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.