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About This Item
UNSPSC Code:
41106514
Biological source:
human cervix
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46
Morphology:
Epithelial-like
biological source
human cervix
packaging
tube of 5 μg 84121902-DNA-5UG, pkg of vial of cells 84121902-1VL
growth mode
Adherent
karyotype
2n = 46
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Transformation studies
Biochem/physiol Actions
Human cervix carcinoma
This was originally a clone of the MRC-5 cell line. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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