Hep2 (Clone 2B) (HeLa derivative)

Tumourigenicity and virus studies: arboviruses, measles, poliovirus 1, Ad 3 and VSV

Human cervix carcinoma

This cell line has been adapted for growth in suspension. Cells support growth of arboviruses, measles virus, poliovirus 1, adenovirus 3 and VSV (Indiana strain). This cell line has been found to be indistinguishable from Hela by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C. Cells grow in suspension and may form clusters.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.