biological source
human blood
packaging
tube of 5 μg 85011413-DNA-5UG, pkg of vial of cells 85011413-1VL
growth mode
Suspension
karyotype
Modal no. 95; hypertetraploid
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
A suspension culture derived from the peripheral blood of a 19 year old male with acute lymphoblastic leukaemia in relapse. A stable T-cell leukaemia that forms rosettes with sheep erythrocytes.
Human acute T lymphoblastic leukaemia
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.