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About This Item
UNSPSC Code:
41106514
Biological source:
mouse connective tissue
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast
biological source
mouse connective tissue
packaging
tube of 5 μg 85011432-DNA-5UG, pkg of vial of cells 85011432-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Somatic cell hybridisation and gene transfer studies
Biochem/physiol Actions
Mouse C34/An connective tissue
Sub-line of BUdR resistant strain of the L-M mouse line (Sigma Catalogue number. 87032401), which in turn was derived from L929 (Sigma Catalogue number. 85011425). TK- cells cannot grow in HAT medium. Stable TK- mutants are only maintained if the cells are kept for several passages in growth medium containing BUdR.
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
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