Skip to Content
Merck
CN
Sign Into View Organizational & Contract Pricing

About This Item

UNSPSC Code:
41106514
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

hamster kidney

packaging

tube of 5 μg 85011433-DNA-5UG
pkg of vial of cells 85011433-1VL

growth mode

Adherent

karyotype

2n = 44, pseudodiploid

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Hamster Syrian kidney

Cell Line Description

Subclone of parent line derived from 5 1-day-old unsexed hamster kidneys. Used extensively for virus replication studies i.e. poliovirus, rabies, foot and mouth disease, VSV (Indiana strain), herpes simplex, Ad25 and arboviruses.

Application

BHK 21 (Clone 13) from hamster has been used in viral transfection studies.
Virus replication studies

Culture Medium

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service