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Merck
CN

B12

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
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biological source

rat nerve

packaging

tube of 5 μg 85042303-DNA-5UG
pkg of vial of cells 85042303-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Glial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Neurobiological studies

Biochem/physiol Actions

Ethyl nitrosourea induced tumour cell line. Glial cells are useful in the research of the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia.
Rat nervous tissue glial

Preparation Note

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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