biological source
rat nerve
packaging
tube of 5 μg 85042303-DNA-5UG, pkg of vial of cells 85042303-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Glial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Neurobiological studies
Biochem/physiol Actions
Ethyl nitrosourea induced tumour cell line. Glial cells are useful in the research of the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia.
Rat nervous tissue glial
Preparation Note
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.