biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 85060404-DNA-5UG, pkg of vial of cells 85060404-1VL
ImmunoDon
AO rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-Phytochrome
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Phytochrome, purified from dark-grown Avena Sativa l seedlings
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat AO spleen
The antibody is directed against phytochrome from Avena. It shows preferential binding to the red-absorbing form of phytochrome.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.