F9
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
mouse embryo
packaging
tube of 5 μg 85061803-DNA-5UG
pkg of vial of cells 85061803-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Differentiation studies
Biochem/physiol Actions
Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cAMP.
Mouse 129 embryo teratocarcinoma
Preparation Note
DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2, 37°C. Upon resuscitation, cells shed large quantities of debris, leave for 2 days before subculture. Please note: Culture v
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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