Mycl-9E10
Reactivity: Anti-c-Myc gene product, human, 85102202, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 85102202-DNA-5UG
pkg of vial of cells 85102202-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-c-Myc gene product, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG1, kappa
shipped in
dry ice
storage temp.
−196°C
Immunogen
A synthetic peptide, corresponding to residues 408-432(C-terminus of Peptide G) of human p62c-myc sequence, conjugated to KLH
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
The antibody recognises human p62c-myc gene product and is specific for C-terminal peptide G. The antibody failed to immunoprecipitate p62 c-myc, but recognised the imunoblotted protein. Compared to other hybridomas specific to this antigen (Mycl-8F9, Mycl-3C7, CT9-B7, CT14-B2) Mycl-9E10 is the most sensitive probe for immunoblots. Conformation of antigen is critical for antibody recognition. No reported cross-reaction, with chicken p110gag-myc protein or mouse c-myc protein. This cell line is available as a growing culture only to customers in Europe.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells are slow to grow. This cell line is available as a growing culture only to customers in Europe.
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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