biological source
Mouse x Rat Heterohybridoma
packaging
tube of 5 μg 85103103-DNA-5UG, pkg of vial of cells 85103103-1VL
ImmunoDon
LOU/M rat spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-Transferrin Receptor, mouse
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Immunogen
The cytolytic T cell clone A15.1.17
Application
The isotype of the monoclonal antibody produced by this cell line is rat IgG2b.
Biochem/physiol Actions
Mouse P3X63Ag8.653 x Rat LOU/M spleen
The antibody is directed against the murine transferrin receptor.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS). Immediately post resuscitation of the cells use media containing 20% FBS, once the culture is established the level of FBS can then be reduced to 10% FBS.
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.