biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 85103106-DNA-5UG, pkg of vial of cells 85103106-1VL
ImmunoDon
A.TH (Ks Is Dd) mouse spleen
Myeloma
NS1
Reactivity
Anti-I-Ek
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a, kappa
shipped in
dry ice
storage temp.
−196°C
Immunogen
A.TL (Ks Ik Dd) mouse spleen cells and thymocytes
Biochem/physiol Actions
Mouse NS1 x Mouse A.TH (Ks Is Dd) spleen
The antibody is directed against the β chain of the I-Ek molecule.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder cells upon resuscitation
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.