biological source
rat blood
packaging
tube of 5 μg 85110501-DNA-5UG, pkg of vial of cells 85110501-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hybridisation and cloning studies
Biochem/physiol Actions
Rat myeloma
The full designation of this line is YB2/3.0Ag30. YO was derived from a hybrid myeloma YB2/3HL by cloning in soft agar to select a non-secreting sub-population. The cells are azaguanine resistant and do not secrete IgG.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.