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About This Item
UNSPSC Code:
41106514
Biological source:
human cervix
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 55
Morphology:
Epithelial
biological source
human cervix
packaging
tube of 5 μg 85112701-DNA-5UG, pkg of vial of cells 85112701-1VL
growth mode
Adherent
karyotype
2n = 55
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Transformation studies
Biochem/physiol Actions
Human cervix carcinoma
This cell line has been shown to be resistant to 8-azaguanine. The cell line was recloned in the depositor′s laboratory and is useful in transformation studies. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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