biological source
mouse blood
packaging
tube of 5 μg 85121301-DNA-5UG, pkg of vial of cells 85121301-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Interleukin 2 (IL-2), Interleukin 4 (IL-4), Thy-1.2
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Resistance and hormone studies
Biochem/physiol Actions
Derivative of EL4.BU. Resistant to BUdR and ouabain and expresses Thy-1.2 Ag. Will die in HAT selective medium. A high producer of interleukin 2 and interleukin 4.
Mouse C57BL/6N ascites lymphoma lymphoblast
Preparation Note
DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 1.16g/l 1% L-Arginine + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.