biological source
mouse embryo
packaging
tube of 5 μg 86060301-DNA-5UG, pkg of vial of cells 86060301-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: N- and B- trophic viruses, MuLV
Biochem/physiol Actions
Mouse feral embryo
Sensitive to both N- and B- trophic viruses. Cells exhibit a greater sensitivity than other mouse lines to MuLV strains and to a limited extent support the replication of xenotropic MuLV. This cell line has been mycoplasma eradicated at ECACC.
Preparation Note
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.