Skip to Content
Merck
CN
Sign Into View Organizational & Contract Pricing

About This Item

UNSPSC Code:
41106514
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

human liver

packaging

tube of 5 μg 86062703-DNA-5UG
pkg of vial of cells 86062703-1VL

growth mode

Adherent

karyotype

2n = 46, modal no. 60

morphology

Epithelial

products

Alpha-foetoproteins, Hepatitis B Surface Antigen (HBSAg), albumin, transferrin, fibrinogen, complement (C3), alpha-2-macroglobulins

receptors

Not specified

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Description

Derived from an 8 year old male. Cells contain integrated Hepatitis B virus genome. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17

Preparation Note

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service