biological source
Human x Mouse Hybridoma unknown/unspecified
packaging
tube of 5 μg 86072805-DNA-5UG, pkg of vial of cells 86072805-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Cystic fibrosis studies, diagnostic assays
Biochem/physiol Actions
Mouse/human hybrid
The hybrid cell line II b has been established by transforming mouse NIH 3T3 (ECACC catalogue no. 93061524) with mitotic chromosomes from the human cell line MNNG-HOS (Sigma Catalogue number. 87070201). Various probes showed stable integration of chromosome 7 DNA (7q22-31.3 and 7q22-7 cen) being closely linked to the gene mutation causing cystic fibrosis
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.