biological source
human eye
packaging
tube of 5 μg 86093003-DNA-5UG, pkg of vial of cells 86093003-1VL
growth mode
Suspension
karyotype
2n = 46, hypertriploid
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established from a primary tumour of the right eye of a 2.5 year old female Caucasian. The cells grow in clusters and exhibit reverse transcriptase activity.
Human Caucasian retinoblastoma
Preparation Note
Maintain cultures between 4-9x100,000 cells/ml; 5% CO2; 37°C.When in culture, stand flask up until cells show growth in large clumps. After approximately 3 days the flask can be laid down.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.