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Merck
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HT 1197

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87032403, human bladder, Epithelial

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About This Item

UNSPSC Code:
41106514
Biological source:
human bladder
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46
Morphology:
Epithelial

Key Documents

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biological source

human bladder

packaging

tube of 5 μg 87032403-DNA-5UG, pkg of vial of cells 87032403-1VL

growth mode

Adherent

karyotype

2n = 46

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

HT 1197 has been used to study the transcription activity of human endogenous retroviruses (HERV).

Biochem/physiol Actions

Derived from a grade 4 transitional cell carcinoma of the bladder of a 44 year old Caucasian male who had not received chemo or radiation therapy. The cells grow in soft agar, produce fibrinolytic activity and are tumorigenic in mice and hamsters.
Human Caucasian bladder carcinoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 16,18

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2x10,000 to 5x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

低风险生物材料
常规特殊物品
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