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About This Item
UNSPSC Code:
41106514
Biological source:
human colon (supraclavicular lymph node metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
Modal no. 49, (2n=46)
Morphology:
Epithelial
biological source
human colon (supraclavicular lymph node metastasis)
packaging
tube of 5 μg 87060101-DNA-5UG, pkg of vial of cells 87060101-1VL
growth mode
Adherent
karyotype
Modal no. 49, (2n=46)
morphology
Epithelial
products
Carcinoembryonic antigen (CEA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
Human LoVo cell line is part of the three-dimensional (3D) heterotypic spheroid model developed to assess cancer immunotherapy agents in vitro. It has been used to test the in vitro cytotoxic property and anti-proliferative property of C. lechleri stem bark essential oil.
Tumourigenicity studies
Biochem/physiol Actions
Derived from a metastatic tumour in the left supraclavicular region of a 56 year old male with adenocarcinoma of the colon. The cells produce carcino embryonic antigen (CEA).
Human colon adenocarcinoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13,14
D13S317: 8,11
D16S539: 9,12
D5S818: 11,13
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 17,18
CSF1PO: 11,13,14
D13S317: 8,11
D16S539: 9,12
D5S818: 11,13
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 17,18
Preparation Note
Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
常规特殊物品
低风险生物材料
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Articles
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
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