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About This Item
UNSPSC Code:
41106514
Biological source:
human nose
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46
Morphology:
Epithelial
biological source
human nose
packaging
tube of 5 μg 88031602-DNA-5UG, pkg of vial of cells 88031602-1VL
growth mode
Adherent
karyotype
2n = 46
morphology
Epithelial
products
Mucoid material
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
From the pleural effusion of an extensive malignant tumour of the nasal septum, diagnosed as anaplastic squamous cell carcinoma. Nearly normal karyotype, stability of chromosome number and probable origin from malignant cells make the cell line unique amongst permanent human cell lines. Cells are very small and grow in clusters fusing to form thick layers.
Human nasal septum quasidiploid tumour
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,11
THO1: 6,8
TPOX: 8
vWA: 16,18
CSF1PO: 9,11
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,11
THO1: 6,8
TPOX: 8
vWA: 16,18
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
常规特殊物品
低风险生物材料
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DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
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