biological source
human blood
packaging
tube of 5 μg 88051601-DNA-5UG, pkg of vial of cells 88051601-1VL
growth mode
Aggregates in suspension
karyotype
Not specified
morphology
Lymphoblast
products
Human T Lymphotrohpic Virus 1 (HTLV-1) tat gene
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Clone of C63/CRII-4 derived by fusion of primary umbilical cord blood cells with HTLV-1 producing line from adult T cell leukaemia lymphoma patient. Contain defective HTLV-1 genome. In presence of HIV cells fuse to form syncytia. The cells should be handled under laboratory containment level 2.
Human T cell leukaemia
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells grow in aggregates.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.