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About This Item
UNSPSC Code:
41106514
Biological source:
mouse liver
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
mouse liver
packaging
tube of 5 μg 88111403-DNA-5UG, pkg of vial of cells 88111403-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)
Biochem/physiol Actions
Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).
Mouse C3H/An liver
Preparation Note
NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO2; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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