biological source
human blood
packaging
tube of 5 μg 88120805-DNA-5UG, pkg of vial of cells 88120805-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
HL60 15-12 is a variant of HL60 which is capable of differentiating towards either neutrophils or monocytes with treatment of appropriate agents.
Human Caucasian promyelocytic leukaemia
Preparation Note
Maintain cultures between 2x100,000 to 1,000,000 cells/ml. NB: The cells will differentiate if the culture medium is allowed to become acidic. 5% CO2; 37°C. Freeze in 10% glycerol and 90% FBS.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.