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About This Item
UNSPSC Code:
41106514
Biological source:
human cervix
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, hypodiploid
Morphology:
Epithelial
biological source
human cervix
packaging
tube of 5 μg 89051702-DNA-5UG, pkg of vial of cells 89051702-1VL
growth mode
Adherent
karyotype
2n = 46, hypodiploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Differentiation studies
Biochem/physiol Actions
Established from a cervical carcinoma of a 41 year old Caucasian female. Cells form stratified, epithelial colonies and retain properties of squamous cell differentiation in long term culture. They are morphologically and cytogenetically distinct from C-4 II (Sigma Catalogue number. 88102701). Reported to contain human papilloma virus. Handle as potentially biohazardous material.
Human Caucasian Cervical carcinoma
Preparation Note
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
W aymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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Regulatory Information
低风险生物材料
常规特殊物品
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