WI 26 VA4

Transformation and virus studies: SV40

An SV40 transformed derivative of WI-26, a human diploid cell line derived from embryonic lung tissue of a male Caucasian. The cells have SV40 T-Ag but infectious virus has not been rescued.

Human Caucasian foetal lung, SV40 transformed

DMEM + 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.