McCoy
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90010305, mouse unknown/unspecified, Fibroblast
biological source
mouse unknown/unspecified
packaging
tube of 5 μg 90010305-DNA-5UG
pkg of vial of cells 90010305-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Propagation of Chlamydia spp.
Biochem/physiol Actions
Mouse fibroblast
Originally derived from synovial fluid of a patient suffering from degenerative arthritis. However, subsequent sub-lines shown to be of mouse origin, with some characteristics similar to the strain L line. Cells have been used to propagate Chlamydia spp.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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