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About This Item
UNSPSC Code:
41106514
Biological source:
human skin
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast
biological source
human skin
packaging
tube of 5 μg 90011862-DNA-5UG, pkg of vial of cells 90011862-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
DNA repair studies
Biochem/physiol Actions
Established from a biopsy of a radiosensitive patient. The patient was diagnosed as not having ataxia-telangiectasia, an autosomal recessive disorder where sensitivity to ionising radiation has been documented.
Human skin fibroblast
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
常规特殊物品
低风险生物材料
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