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About This Item
UNSPSC Code:
41106514
Biological source:
human brain
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Astrocytoma
biological source
human brain
packaging
tube of 5 μg 90021502-DNA-5UG, pkg of vial of cells 90021502-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Astrocytoma
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
HLA profiles
Biochem/physiol Actions
Established from a Grade IV astrocytoma from a 68-year old Caucasian female. Cells exhibit strong perinuclear acid and phosphatase activity, glial fibrillary acidic protein present in 70-80% cells. HLA-DR present on approximately 20-30% cells after 48 hours culture.
Human Caucasian astrocytoma
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
常规特殊物品
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