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About This Item
UNSPSC Code:
41106514
Biological source:
human skin
Growth mode:
Adherent
Karyotype:
Diploid
Morphology:
Fibroblast
biological source
human skin
packaging
tube of 5 μg 90031532-DNA-5UG, pkg of vial of cells 90031532-1VL
growth mode
Adherent
karyotype
Diploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Interferon assay
Biochem/physiol Actions
Derived from the normal skin of a 56 year old Caucasian female. The cells are highly sensitive to human interferon and have been proposed as a standard for its assay.
Human Caucasian skin fibroblast
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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