L-M (TK-)
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
mouse connective tissue
packaging
tube of 5 μg 90083001-DNA-5UG
pkg of vial of cells 90083001-1VL
growth mode
Adherent
karyotype
Modal no. 45; hyperdiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Gene transfer, genetic, biochemical and hybridisation studies
Biochem/physiol Actions
Derived from a sub-line of a BUdR resistant strain of the L-M mouse fibroblast cell line. This cell line has potential for use in genetic and biochemical studies including somatic cell hybridisation and gene transfer.
Mouse C34/An connective tissue
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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