biological source
mouse connective tissue
packaging
tube of 5 μg 90083001-DNA-5UG, pkg of vial of cells 90083001-1VL
growth mode
Adherent
karyotype
Modal no. 45; hyperdiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Gene transfer, genetic, biochemical and hybridisation studies
Biochem/physiol Actions
Derived from a sub-line of a BUdR resistant strain of the L-M mouse fibroblast cell line. This cell line has potential for use in genetic and biochemical studies including somatic cell hybridisation and gene transfer.
Mouse C34/An connective tissue
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.