DLD-1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90102540, human colon, Epithelial
biological source
human colon
packaging
tube of 5 μg 90102540-DNA-5UG
pkg of vial of cells 90102540-1VL
growth mode
Adherent
karyotype
2n = 46, pseudodiploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
DLD-1 cell line has been used to express shRNA oligos directed against coronin 2A (CRN5) and to study the role of CRN5 in tumor cell migration. It has also been used to determine the efficiency of antibody-coupled short interfering RNA (siRNA) for in vivo mRNA knockdown.
Biochem/physiol Actions
Derived from human colorectal adenocarcinoma. The cells have been used in the study of polar solvents on cell characteristics.The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.
Human colon adenocarcinoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin, 0.02% EDTA.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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