biological source
human colon
packaging
tube of 5 μg 90102543-DNA-5UG, pkg of vial of cells 90102543-1VL
growth mode
Adherent
karyotype
2n = 46
morphology
Epithelial
products
Carcinoembryonic antigen (CEA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Derived from a grade III adenocarcinoma of the colon in a 53 year old female. CEA is produced and electron microscopy reveals the presence of brush borders around colonies in vitro.
Human colon adenocarcinoma
Preparation Note
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37°C. Centrifugation and resuspension are recommended to disperse clumps.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.