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Merck
CN

KLN 205

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90110519, mouse unknown/unspecified, Not specified

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About This Item

UNSPSC Code:
41106514
Biological source:
mouse unknown/unspecified
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Not specified
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biological source

mouse unknown/unspecified

packaging

tube of 5 μg 90110519-DNA-5UG, pkg of vial of cells 90110519-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Chemotherapeutic agent and tumourigenicity studies

Biochem/physiol Actions

Cells produce metastases in the lungs when inoculated intravenously into DBA/2 or BDF1 mice. The cells are a potential model for chemotherapeutic screening.
Mouse squamous cell carcinoma

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.


Regulatory Information

动物来源培养基

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