Detroit 573

Karyology studies

Detroit 573 was initiated in 1967 from a skin biopsy of a deceased new-born female with multiple congenital anomalies. Approximately 94% of the cell population is 45,XX,t(Bp-;Dq+). The translocated B/D chromosome morphologically resembles a group A-2 chromosome. The cell line is susceptible to polyvirus type 1 and vesicular stomatitis (Indiana Strain) virus.

Human Caucasian skin, B/D translocation

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.