HG261

Chromosome and virus studies: SV40

From 6 year old Caucasian male with Faconi′s anaemia which is associated with high incidence of leukaemia. Chromosomes show a tendency to develop spontaneous abnormalities including breaks and complex rearrangement. Have a higher proportion of transformed colonies with SV40 compared with normal diploid cells.

Human Caucasian skin Fanconi′s anaemia

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Limited lifespan; up to 35 passages possible.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.