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Merck
CN

G-402

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
Biological source:
human kidney
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Modal no. 47
Morphology:
Epithelial-like
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biological source

human kidney

packaging

tube of 5 μg 90112715-DNA-5UG, pkg of vial of cells 90112715-1VL

growth mode

Adherent

karyotype

Modal no. 47

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Transformation and tumourigenicity studies

Biochem/physiol Actions

Derived from a tumour of a 9 month old female Caucasian. Highly transformed and grows in soft agar.
Human Caucasian renal leiomyoblastoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,9
TPOX: 10
vWA: 13,17

Preparation Note

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Change medium every 4 days.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.


Regulatory Information

低风险生物材料
常规特殊物品

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