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About This Item
UNSPSC Code:
41106514
Biological source:
rat lung
Growth mode:
Adherent
Karyotype:
Modal no. 77
Morphology:
Epithelial
biological source
rat lung
packaging
tube of 5 μg 90112811-DNA-5UG, pkg of vial of cells 90112811-1VL
growth mode
Adherent
karyotype
Modal no. 77
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from normal rat lung tissue. The cells possess elevated levels of specific enzymes involved in the synthesis of phosphatidyl choline. Osmiophilic lamellar inclusions are observed in cell pellets from mass culture.
Rat lung
Preparation Note
Coon′s modified Ham′s F12 or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
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