biological source
human unknown/unspecified
packaging
tube of 5 μg 90121003-DNA-5UG, pkg of vial of cells 90121003-1VL
growth mode
Suspension
karyotype
2n = 46
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of Burkitt′s lymphoma, virus sensitivity
Biochem/physiol Actions
Human Burkitt′s lymphoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
THO1: 7
TPOX: 6,9
vWA: 17,19
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
THO1: 7
TPOX: 6,9
vWA: 17,19
The cell line EB3 has been established in 1964 from a 3 year old male with Burkitt′s lymphoma. Herpes-like virus particles have been detected by electron microscopy. Antibodies from patients with infectious mononucleosis react with the herpes type virus (EBV) found in EB3 cells. The cell line has been shown to be resistant to challenge with vesicular stomatitis virus. Lymphoblastoid, grow as single cells and in clumps. Ethnicity: Black.
Preparation Note
Maintain cultures between 3-9 x 100,000 cells / ml; 5% CO2; 37°C
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.