biological source
human blood
packaging
tube of 5 μg 90121307-DNA-5UG, pkg of vial of cells 90121307-1VL
growth mode
Suspension
karyotype
2n = 46
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Transformation and virus studies: EBV
Biochem/physiol Actions
Established by seeding peripheral blood lymphocytes from 20 year old female, 2 months after onset of infectious mononucleosis. EBV transformed B-lymphoblastoid line. The cells should be handled under laboratory containment level 2. Cells are particularly resistant to poliomyelitis and vesicular stomatis, viruses similar to other cell lines carrying EB virus.
Human Caucasian infectious mononucleosis lymphocytes
Preparation Note
Maintain cultures between 4-5x100,000 cells/ml; 5% CO2; 37°C. Subculture every 8 days by centrifugation. Maximun of 1-2 x 1,000,000 viable cells/ml is obtainable.
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.