biological source
hamster peritoneum
packaging
tube of 5 μg 91010303-DNA-5UG, pkg of vial of cells 91010303-1VL
growth mode
Adherent
karyotype
2n = 22; Modal no. 23
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Nutritional studies
Biochem/physiol Actions
A chemically defined medium derivative of B14FAF28-G3. Since it was established, NCTC cells have been employed in nutritional studies involving 6 different NCTC chemically defined serum-free media.
Hamster Chinese peritoneum
Preparation Note
NCTC 135 100% + 2mM Glutamine.
Sub-cultures are prepared by scraping; 5% CO2; 37°C. Media change three times a week. Freeze in 9% glycerol/91% FBS.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.