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biological source

Porcine blood

growth mode

Suspension

karyotype

Modal no. 36.5

morphology

Not specified

products

Immunoglobulin M (IgM)

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Porcine peripheral blood B cell

Cell Line Description

One of a series of cell lines established from mononuclear cells of a boar (Sus scrofa domestica L) which was injected with Shimozuma cells producing porcine retrovirus (Tsukuba-1). Contain retrovirus particles; positive in RT assay.

DNA Profile

Not specified

Culture Medium

RPMI 1640 + 2 mM Glutamine + 1 mM Sodium Pyruvate (NaP) + 0.05 mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO2; 37 °C. Cells grow slowly and in small clumps. Slightly acidic conditions preferred for growth.

Other Notes

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