biological source
mouse blood
packaging
tube of 5 μg 91061231-DNA-5UG, pkg of vial of cells 91061231-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Monocyte
products
Expresses the MAC-1 antigen.
receptors
Fc
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from an Abelson virus induced tumour in a BALB/c mouse. The cells express Mac-1 but not Ia and are not phagocytic.
Mouse monocyte
Preparation Note
DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 1x100,000 to 1x1000,000 cells/ml. Adherent cells can be dislodged by scraping and re-established by centrifugation and subsequent resuspension; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.