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About This Item
UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
human colon
packaging
tube of 5 μg 92012401-DNA-5UG, pkg of vial of cells 92012401-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.
Human Caucasian colon adenocarcinoma
Preparation Note
DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO3) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
常规特殊物品
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