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About This Item
eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
human lung
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
human lung
packaging
tube of 5 μg 92012463-DNA-5UG, pkg of vial of cells 92012463-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Control cell line for heterozygosity studies using the AGLCL cell line
Biochem/physiol Actions
Derived from a lung squamous cell carcinoma of a 72 year old Caucasian male. The B-lymphoblastoid cell line AGLCL (Sigma Catalogue number. 89120566) was derived from the same patient. Cells grow as large swollen aggregates, which will detach and eventually grow in suspension.
Human Caucasian lung squamous cell carcinoma
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
常规特殊物品
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