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U-2 OS Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92022711, human bone, Not specified

Synonym(s):

U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

human bone

packaging

tube of 5 μg 92022711-DNA-5UG
pkg of vial of cells 92022711-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

U-2 OS has been used to study:
  • the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
  • the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
  • calcium-mediated actin reset (CaAR) in response to physiological changes

Biochem/physiol Actions

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.
Human Osteosarcoma
STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

Preparation Note

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).
Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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Articles

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Cell cycle regulates vital processes like DNA repair, cancer prevention. Four stages: G1, S, G2, M. NTPs don't permeate membranes.

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